› 6x6o

X-ray diffraction
1.52Å resolution

Crystal structure of T4 protein Spackle as determined by native SAD phasing

Released:
DOI: 10.2210/pdb6x6o/pdb
PDB entry 6x6o coloured by chain, front view.
PDB entry 6x6o coloured by chain, side view.
PDB entry 6x6o coloured by chain, top view.
Source organism: Escherichia virus T4
Primary publication:
Crystal structure of bacteriophage T4 Spackle as determined by native SAD phasing.
Shi K, Kurniawan F, Banerjee S, Moeller NH, Aihara H
Acta Crystallogr D Struct Biol 76 899-904 (2020)
PMID: 32876065

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153854 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein spackle Chains: A, B
Molecule details ›
Chains: A, B
Length: 105 amino acids
Theoretical weight: 12.08 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P39230 (Residues: 1-97; Coverage: 100%)
Gene names: 61.3, sp

Ligands and Environments

1 bound ligand:
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: I222
Unit cell:
a: 44.424Å b: 79.822Å c: 93.227Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.173 0.191
Expression system: Escherichia coli

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Citations

1 review citation

Glycoside hydrolases in the biodegradation of lignocellulosic biomass.
Lu et al. (2023)
2 other articles

3 mentions without citation

Implications of AlphaFold2 for crystallographic phasing by molecular replacement.
McCoy et al. (2022)
2 more