› 6wlh

Solution NMR

RNA complex of WT1 zinc finger transcription factor

Released:
DOI: 10.2210/pdb6wlh/pdb
PDB entry 6wlh coloured by chain, ensemble of 20 models, front view.
PDB entry 6wlh coloured by chain, ensemble of 20 models, side view.
PDB entry 6wlh coloured by chain, ensemble of 20 models, top view.
Source organisms:
Primary publication:
RNA Binding by the KTS Splice Variants of Wilms' Tumor Suppressor Protein WT1.
Nishikawa T, Wojciak JM, Dyson HJ, Wright PE
Biochemistry 59 3889-3901 (2020)
PMID: 32955251

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148638 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Wilms tumor protein Chain: A
Molecule details ›
Chain: A
Length: 118 amino acids
Theoretical weight: 14.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19544 (Residues: 318-438; Coverage: 26%)
Gene name: WT1
Sequence domains: Zinc finger, C2H2 type
RNA (29-MER) Chain: B
Molecule details ›
Chain: B
Length: 29 nucleotides
Theoretical weight: 9.3 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 39%
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 review citation

The function of alternative splicing in the proteome: rewiring protein interactomes to put old functions into new contexts.
Kjer-Hansen et al. (2023)

1 mention without citation

RNA Binding by the KTS Splice Variants of Wilms' Tumor Suppressor Protein WT1.
Nishikawa et al. (2020)