› 6wf3

X-ray diffraction
2.29Å resolution

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + an N-terminal-L-methionyl-L-alanyl-[protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-L-alanyl-[protein] + CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167701 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
N-alpha-acetyltransferase 50 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 171 amino acids
Theoretical weight: 19.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9GZZ1 (Residues: 1-169; Coverage: 100%)
Gene names: MAK3, NAA50, NAT13, NAT5
Sequence domains: Acetyltransferase (GNAT) family
ACE-MET-LEU-GLY-PRO-NH2 Chains: G, H, I, J, K, L
Molecule details ›
Chains: G, H, I, J, K, L
Length: 6 amino acids
Theoretical weight: 441 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments


Cofactor: Ligand COA 6 x COA
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 48.547Å b: 124.17Å c: 109.085Å
α: 90° β: 100.92° γ: 90°
R-values:
R R work R free
0.21 0.208 0.243
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

DNA-Encoded Chemistry: Drug Discovery from a Few Good Reactions.
Fitzgerald et al. (2021)
3 more

1 mention without citation

Characterization of Specific N-α-Acetyltransferase 50 (Naa50) Inhibitors Identified Using a DNA Encoded Library.
Kung et al. (2020)