› 6wcy

X-ray diffraction
1.2Å resolution

N160D Deamidation Mutant of Human gammaD-Crystallin

Released:
DOI: 10.2210/pdb6wcy/pdb
PDB entry 6wcy coloured by chain, front view.
PDB entry 6wcy coloured by chain, side view.
PDB entry 6wcy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Assessing the Structures and Interactions of γD-Crystallin Deamidation Variants.
Guseman AJ, Whitley MJ, González JJ, Rathi N, Ambarian M, Gronenborn AM
Structure 29 284-291.e3 (2021)
PMID: 33264606

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139502 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gamma-crystallin D Chains: A, B
Molecule details ›
Chains: A, B
Length: 173 amino acids
Theoretical weight: 20.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P07320 (Residues: 2-174; Coverage: 99%)
Gene names: CRYG4, CRYGD
Sequence domains: Beta/Gamma crystallin

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 50.811Å b: 51.872Å c: 113.991Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.169 0.194
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Chemical Properties Determine Solubility and Stability in βγ-Crystallins of the Eye Lens.
Rocha et al. (2021)
1 more

1 mention without citation

Assessing the Structures and Interactions of γD-Crystallin Deamidation Variants.
Guseman et al. (2021)