› 6wc5

X-ray diffraction
2.9Å resolution

Crystal Structure of a Ternary MEF2B/NKX2-5/myocardin enhancer DNA Complex

Released:
DOI: 10.2210/pdb6wc5/pdb
PDB entry 6wc5 coloured by chain, front view.
PDB entry 6wc5 coloured by chain, side view.
PDB entry 6wc5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal Structures of Ternary Complexes of MEF2 and NKX2-5 Bound to DNA Reveal a Disease Related Protein-Protein Interaction Interface.
Lei X, Zhao J, Sagendorf JM, Rajashekar N, Xu J, Dantas Machado AC, Sen C, Rohs R, Feng P, Chen L
J Mol Biol 432 5499-5508 (2020)
PMID: 32681840

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120823 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Myocyte-specific enhancer factor 2B Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 90 amino acids
Theoretical weight: 10.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02080 (Residues: 2-91; Coverage: 25%)
Gene names: MEF2B, XMEF2
Sequence domains: SRF-type transcription factor (DNA-binding and dimerisation domain)
Homeobox protein Nkx-2.5 Chains: I, N
Molecule details ›
Chains: I, N
Length: 57 amino acids
Theoretical weight: 7.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P52952 (Residues: 140-196; Coverage: 18%)
Gene names: CSX, NKX2-5, NKX2.5, NKX2E
Sequence domains: Homeodomain
Myocardin enhancer DNA Chains: E, G
Molecule details ›
Chains: E, G
Length: 22 nucleotides
Theoretical weight: 6.77 KDa
Myocardin enhancer DNA Chains: F, H
Molecule details ›
Chains: F, H
Length: 22 nucleotides
Theoretical weight: 6.73 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 66.04Å b: 93.24Å c: 136.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.251
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

The benign nature and rare occurrence of cardiac myxoma as a possible consequence of the limited cardiac proliferative/ regenerative potential: a systematic review.
Shafi et al. (2023)

1 mention without citation

Crystal Structures of Ternary Complexes of MEF2 and NKX2-5 Bound to DNA Reveal a Disease Related Protein-Protein Interaction Interface.
Lei et al. (2020)