› 6w0a

X-ray diffraction
3.24Å resolution

Open-gate KcsA soaked in 1 mM BaCl2

Released:
DOI: 10.2210/pdb6w0a/pdb
PDB entry 6w0a coloured by chain, front view.
PDB entry 6w0a coloured by chain, side view.
PDB entry 6w0a coloured by chain, top view.
Source organisms:
Primary publication:
Open and Closed Structures of a Barium-Blocked Potassium Channel.
Rohaim A, Gong L, Li J, Rui H, Blachowicz L, Roux B
J Mol Biol 432 4783-4798 (2020)
PMID: 32615129

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-231722 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab Heavy Chain Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 23.41 KDa
Source organism: Rattus norvegicus
Expression system: Homo sapiens
Fab Light Chain Chain: B
Molecule details ›
Chain: B
Length: 212 amino acids
Theoretical weight: 23.44 KDa
Source organism: Rattus norvegicus
Expression system: Homo sapiens
pH-gated potassium channel KcsA Chain: C
Molecule details ›
Chain: C
Length: 93 amino acids
Theoretical weight: 9.73 KDa
Source organism: Streptomyces lividans
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A334 (Residues: 28-120; Coverage: 58%)
Gene names: kcsA, skc1
Sequence domains: Ion channel

Ligands and Environments

2 bound ligands:
Ligand BA 1 x BA
Ligand K 1 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: I4
Unit cell:
a: 156.569Å b: 156.569Å c: 73.895Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.18 0.242
Expression systems:
  • Homo sapiens
  • Escherichia coli

Quick links

Citations

5 citation in other articles

Non-autonomous stomatal control by pavement cell turgor via the K+ channel subunit AtKC1.
Nieves-Cordones et al. (2022)
4 more

3 mentions without citation

Open and Closed Structures of a Barium-Blocked Potassium Channel.
Rohaim et al. (2020)
2 more