› 6vpi

X-ray diffraction
2Å resolution

TPX2 residues 7-20 fused to Aurora A residues 116-389 C247V + D256N + C319V triple mutant disulfide homodimer in complex with AMP-PNP

Released:
DOI: 10.2210/pdb6vpi/pdb
PDB entry 6vpi coloured by chain, front view.
PDB entry 6vpi coloured by chain, side view.
PDB entry 6vpi coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Redox priming promotes Aurora A activation during mitosis.
Lim DC, Joukov V, Rettenmaier TJ, Kumagai A, Dunphy WG, Wells JA, Yaffe MB
Sci Signal 13 (2020)
PMID: 32694171

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127231 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aurora kinase A; Targeting protein for Xklp2 Chain: A
Molecule details ›
Chain: A
Length: 292 amino acids
Theoretical weight: 33.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O14965 (Residues: 104-104, 117-389; Coverage: 68%)
  • Canonical: Q9ULW0 (Residues: 8-21; Coverage: 2%)
Gene names: AIK, AIRK1, ARK1, AURA, AURKA, AYK1, BTAK, C20orf1, C20orf2, DIL2, HCA519, IAK1, STK15, STK6, TPX2
Sequence domains: Protein kinase domain

Ligands and Environments

3 bound ligands:
Ligand ANP 1 x ANP
Ligand MG 2 x MG
Ligand MLA 1 x MLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P41212
Unit cell:
a: 85.292Å b: 85.292Å c: 128.246Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.215
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Effects of aneuploidy on cell behaviour and function.
Li et al. (2022)
4 more

1 mention without citation

Structure and dynamics of the ASB9 CUL-RING E3 Ligase.
Lumpkin et al. (2020)