› 6vjl

X-ray diffraction
1.3Å resolution

Streptavidin mutant M112 (G26C/A46C)

Released:
DOI: 10.2210/pdb6vjl/pdb
PDB entry 6vjl coloured by chain, front view.
PDB entry 6vjl coloured by chain, side view.
PDB entry 6vjl coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
Engineering a disulfide-gated switch in streptavidin enables reversible binding without sacrificing binding affinity.
Marangoni JM, Wu SC, Fogen D, Wong SL, Ng KKS
Sci Rep 10 12483 (2020)
PMID: 32719366

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chain: A
Molecule details ›
Chain: A
Length: 160 amino acids
Theoretical weight: 16.71 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22629 (Residues: 25-183; Coverage: 100%)
Sequence domains: Avidin family

Ligands and Environments

1 bound ligand:
Ligand BTN 1 x BTN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: I4122
Unit cell:
a: 57.772Å b: 57.772Å c: 184.419Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.19 0.201
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

Engineering a disulfide-gated switch in streptavidin enables reversible binding without sacrificing binding affinity.
Marangoni et al. (2020)