› 6vco

X-ray diffraction
1.7Å resolution

Crystal structure of E.coli RppH in complex with ppcpA

Released:
DOI: 10.2210/pdb6vco/pdb
PDB entry 6vco coloured by chain, front view.
PDB entry 6vco coloured by chain, side view.
PDB entry 6vco coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Principles of RNA and nucleotide discrimination by the RNA processing enzyme RppH.
Gao A, Vasilyev N, Kaushik A, Duan W, Serganov A
Nucleic Acids Res 48 3776-3788 (2020)
PMID: 31960065

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141544 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA pyrophosphohydrolase Chain: A
Molecule details ›
Chain: A
Length: 161 amino acids
Theoretical weight: 18.97 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A776 (Residues: 1-160; Coverage: 91%)
Gene names: JW2798, b2830, nudH, rppH, ygdP
Sequence domains: NUDIX domain

Ligands and Environments

1 bound ligand:
Ligand APC 1 x APC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: C2
Unit cell:
a: 79.14Å b: 38.955Å c: 57.734Å
α: 90° β: 100.064° γ: 90°
R-values:
R R work R free
0.173 0.172 0.203
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

The stringent response and physiological roles of (pp)pGpp in bacteria.
Irving et al. (2021)
3 other articles

1 mention without citation

Principles of RNA and nucleotide discrimination by the RNA processing enzyme RppH.
Gao et al. (2020)