› 6vac

Electron Microscopy
5.7Å resolution

Mouse retromer (VPS26/VPS35/VPS29) heterotrimer

Released:
DOI: 10.2210/pdb6vac/pdb
PDB entry 6vac coloured by chain, front view.
PDB entry 6vac coloured by chain, side view.
PDB entry 6vac coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Mammalian Retromer Is an Adaptable Scaffold for Cargo Sorting from Endosomes.
Kendall AK, Xie B, Xu P, Wang J, Burcham R, Frazier MN, Binshtein E, Wei H, Graham TR, Nakagawa T, Jackson LP
Structure 28 393-405.e4 (2020)
PMID: 32027819
Related structures: EMD-21136

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-154075 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Vacuolar protein sorting-associated protein 35 Chain: A
Molecule details ›
Chain: A
Length: 796 amino acids
Theoretical weight: 91.82 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9EQH3 (Residues: 1-796; Coverage: 100%)
Gene names: Mem3, Vps35
Sequence domains: Vacuolar protein sorting-associated protein 35
Vacuolar protein sorting-associated protein 26A Chain: B
Molecule details ›
Chain: B
Length: 327 amino acids
Theoretical weight: 38.17 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P40336 (Residues: 1-327; Coverage: 100%)
Gene names: Vps26, Vps26a
Sequence domains: Vacuolar protein sorting-associated protein 26
Vacuolar protein sorting-associated protein 29 Chain: C
Molecule details ›
Chain: C
Length: 182 amino acids
Theoretical weight: 20.52 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9QZ88 (Residues: 1-182; Coverage: 100%)
Gene name: Vps29
Sequence domains: Calcineurin-like phosphoesterase superfamily domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 5.7Å
Relevant EMDB volumes: EMD-21136
Expression system: Escherichia coli

Quick links

Citations

7 review citations

The cell biology of Parkinson's disease.
Panicker et al. (2021)
6 more

3 mentions without citation

Understanding the contributions of VPS35 and the retromer in neurodegenerative disease.
Williams et al. (2022)
2 more