› 6uts

X-ray diffraction
3.09Å resolution

Crystal Structure of bacterial pirin YhhW in complex with nickel(II) from Escherichia coli

Released:
DOI: 10.2210/pdb6uts/pdb
PDB entry 6uts coloured by chain, front view.
PDB entry 6uts coloured by chain, side view.
PDB entry 6uts coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structure-Dependent Modulation of Substrate Binding and Biodegradation Activity of Pirin Proteins toward Plant Flavonols.
Guo B, Zhang Y, Hicks G, Huang X, Li R, Roy N, Jia Z
ACS Chem Biol 14 2629-2640 (2019)
PMID: 31609578

Function and Biology Details

Reaction catalysed: 
Quercetin + O(2) = 2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate + CO + H(+)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155520 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Quercetin 2,3-dioxygenase Chain: A
Molecule details ›
Chain: A
Length: 231 amino acids
Theoretical weight: 26.31 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P46852 (Residues: 1-231; Coverage: 100%)
Gene names: JW3402, b3439, yhhW
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand NI 1 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P43212
Unit cell:
a: 108.87Å b: 108.87Å c: 83.368Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.23 0.265
Expression system: Escherichia coli

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Citations

6 citation in other articles

Hydroxylation decoration patterns of flavonoids in horticultural crops: chemistry, bioactivity and biosynthesis.
Liu et al. (2022)
5 more