› 6usu

X-ray diffraction
2.09Å resolution

Crystal structure of GluN1/GluN2A ligand-binding domain in complex with L689,560 and glutamate

Released:
DOI: 10.2210/pdb6usu/pdb
PDB entry 6usu coloured by chain, front view.
PDB entry 6usu coloured by chain, side view.
PDB entry 6usu coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structural Basis of Functional Transitions in Mammalian NMDA Receptors.
Chou TH, Tajima N, Romero-Hernandez A, Furukawa H
Cell 182 357-371.e13 (2020)
PMID: 32610085

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152907 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Glutamate receptor ionotropic, NMDA 1 Chain: A
Molecule details ›
Chain: A
Length: 292 amino acids
Theoretical weight: 33.34 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P35439 (Residues: 394-544, 663-800; Coverage: 31%)
Gene names: Grin1, Nmdar1
Sequence domains:
Glutamate receptor ionotropic, NMDA 2A Chain: B
Molecule details ›
Chain: B
Length: 283 amino acids
Theoretical weight: 31.79 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q00959 (Residues: 402-539, 661-802; Coverage: 19%)
Gene name: Grin2a
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand QGM 1 x QGM
Ligand GLU 1 x GLU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 54.04Å b: 87.341Å c: 136.987Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.189 0.222
Expression system: Escherichia coli K-12

Quick links

Citations

8 review citations

Structure, Function, and Pharmacology of Glutamate Receptor Ion Channels.
Hansen et al. (2021)
7 more

1 mention without citation

Structural Basis of Functional Transitions in Mammalian NMDA Receptors.
Chou et al. (2020)