› 6uoe

X-ray diffraction
1.8Å resolution

3-25 Fab germline-reversion variant bound to an HCMV gB-derived peptide

Released:
DOI: 10.2210/pdb6uoe/pdb
PDB entry 6uoe coloured by chain, front view.
PDB entry 6uoe coloured by chain, side view.
PDB entry 6uoe coloured by chain, top view.
Source organisms:
Primary publication:
Recognition of a highly conserved glycoprotein B epitope by a bivalent antibody neutralizing HCMV at a post-attachment step.
Ye X, Su H, Wrapp D, Freed DC, Li F, Yuan Z, Tang A, Li L, Ku Z, Xiong W, Jaijyan D, Zhu H, Wang D, McLellan JS, Zhang N, Fu TM, An Z
PLoS Pathog 16 e1008736 (2020)
PMID: 32745149

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-139055 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Immunoglobulin gamma-1 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 229 amino acids
Theoretical weight: 24.38 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P0DOX5 (Residues: 1-98, 99-107, 108-223; Coverage: 50%)
Sequence domains:
Immunoglobulin kappa light chain Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.51 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P0DOX7 (Residues: 1-97, 98-214; Coverage: 100%)
Sequence domains:
Envelope glycoprotein B Chain: P
Molecule details ›
Chain: P
Length: 15 amino acids
Theoretical weight: 1.78 KDa
Source organism: Human betaherpesvirus 5
Expression system: Not provided
UniProt:
  • Canonical: P06473 (Residues: 65-79; Coverage: 2%)
Gene names: UL55, gB

Ligands and Environments

2 bound ligands:
Ligand TRS 1 x TRS
Ligand PEG 1 x PEG
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 118.663Å b: 67.587Å c: 84.031Å
α: 90° β: 133.12° γ: 90°
R-values:
R R work R free
0.162 0.16 0.186
Expression systems:
  • Homo sapiens
  • Not provided

Quick links

Citations

1 review citation

Computational identification of HCV neutralizing antibodies with a common HCDR3 disulfide bond motif in the antibody repertoires of infected individuals.
Bozhanova et al. (2022)
8 other articles

2 mentions without citation

A single, improbable B cell receptor mutation confers potent neutralization against cytomegalovirus.
Jenks et al. (2023)
1 more