› 6unb

X-ray diffraction
1.3Å resolution

Crystal structure of CTX-M-14 in complex with temocillin

Released:
DOI: 10.2210/pdb6unb/pdb
PDB entry 6unb coloured by chain, front view.
PDB entry 6unb coloured by chain, side view.
PDB entry 6unb coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Influence of the α-Methoxy Group on the Reaction of Temocillin with Pseudomonas aeruginosa PBP3 and CTX-M-14 β-Lactamase.
Sacco MD, Kroeck KG, Kemp MT, Zhang X, Andrews LD, Chen Y
Antimicrob Agents Chemother 64 (2019)
PMID: 31685462

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192211 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments

2 bound ligands:
Ligand TJ7 2 x TJ7
Ligand PO4 4 x PO4
1 modified residue:
Ligand PCA 2 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P21
Unit cell:
a: 45.11Å b: 106.46Å c: 47.67Å
α: 90° β: 101.92° γ: 90°
R-values:
R R work R free
0.127 0.125 0.167
Expression system: Escherichia coli

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Citations

3 citation in other articles

Reduced In Vitro Susceptibility of Streptococcus pyogenes to β-Lactam Antibiotics Associated with Mutations in the pbp2x Gene Is Geographically Widespread.
Musser et al. (2020)
2 more

1 mention without citation

Influence of the α-Methoxy Group on the Reaction of Temocillin with Pseudomonas aeruginosa PBP3 and CTX-M-14 β-Lactamase.
Sacco et al. (2019)