› 6u82

X-ray diffraction
3.21Å resolution

Crystal Structure of the Double Homeodomain of DUX4 in Complex with a DNA aptamer containing bulge and loop

Released:
DOI: 10.2210/pdb6u82/pdb
PDB entry 6u82 coloured by chain, front view.
PDB entry 6u82 coloured by chain, side view.
PDB entry 6u82 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
DNA aptamers against the DUX4 protein reveal novel therapeutic implications for FSHD.
Klingler C, Ashley J, Shi K, Stiefvater A, Kyba M, Sinnreich M, Aihara H, Kinter J
FASEB J 34 4573-4590 (2020)
PMID: 32020675

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120677 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Double homeobox protein 4 Chains: A, D
Molecule details ›
Chains: A, D
Length: 134 amino acids
Theoretical weight: 15.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UBX2 (Residues: 17-150; Coverage: 32%)
Gene names: DUX10, DUX4
Sequence domains: Homeodomain
DNA (38-MER) Chains: B, E
Molecule details ›
Chains: B, E
Length: 38 nucleotides
Theoretical weight: 11.64 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-E
Spacegroup: C2
Unit cell:
a: 120.32Å b: 75.67Å c: 82.69Å
α: 90° β: 121.6° γ: 90°
R-values:
R R work R free
0.253 0.252 0.28
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

10 review citations

Facioscapulohumeral muscular dystrophy: genetics, gene activation and downstream signalling with regard to recent therapeutic approaches: an update.
Schätzl et al. (2021)
9 more

2 mentions without citation

Modelling aptamers with nucleic acid mimics (NAM): From sequence to three-dimensional docking.
Oliveira et al. (2022)
1 more