› 6swh

X-ray diffraction
2.8Å resolution

Crystal structure of the ternary complex between the type 1 pilus proteins FimC, FimI and FimA from E. coli

Released:
DOI: 10.2210/pdb6swh/pdb
PDB entry 6swh coloured by chain, front view.
PDB entry 6swh coloured by chain, side view.
PDB entry 6swh coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Giese C, Puorger C, Ignatov O, Weber M, Scharer MA, Capitani G, Glockshuber R

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-137419 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Chaperone protein FimC Chains: A, D
Molecule details ›
Chains: A, D
Length: 205 amino acids
Theoretical weight: 22.75 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P31697 (Residues: 37-241; Coverage: 100%)
Gene names: JW4279, b4316, fimC
Sequence domains:
Fimbrin-like protein FimI Chains: B, E
Molecule details ›
Chains: B, E
Length: 160 amino acids
Theoretical weight: 17.19 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P39264 (Residues: 20-179; Coverage: 100%)
Gene names: JW5779, b4315, fimI
Sequence domains: Fimbrial protein
Type-1 fimbrial protein, A chain Chains: C, F
Molecule details ›
Chains: C, F
Length: 153 amino acids
Theoretical weight: 15.51 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P04128 (Residues: 37-182; Coverage: 92%)
Gene names: JW4277, b4314, fimA, pilA
Sequence domains: Fimbrial protein

Ligands and Environments

2 bound ligands:
Ligand PEG 1 x PEG
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P61
Unit cell:
a: 198.35Å b: 198.35Å c: 87.56Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.171 0.17 0.207
Expression system: Escherichia coli

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