› 6stz

X-ray diffraction
1.14Å resolution

Crystal structure of dimethylated RSLex - cucurbituril free form

Released:
DOI: 10.2210/pdb6stz/pdb
PDB entry 6stz coloured by chain, front view.
PDB entry 6stz coloured by chain, side view.
PDB entry 6stz coloured by chain, top view.
Source organism: Ralstonia solanacearum
Primary publication:
Engineered assembly of a protein-cucurbituril biohybrid.
Guagnini F, Engilberge S, Ramberg KO, Pérez J, Crowley PB
Chem Commun (Camb) 56 360-363 (2020)
PMID: 31825399

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186920 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fucose-binding lectin protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 89 amino acids
Theoretical weight: 9.85 KDa
Source organism: Ralstonia solanacearum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8XXK6 (Residues: 3-91; Coverage: 98%)
Gene names: RSc2107, rsl
Sequence domains: Fungal fucose-specific lectin

Ligands and Environments

1 bound ligand:
Ligand BDF 12 x BDF
1 modified residue:
Ligand MLY 24 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P32
Unit cell:
a: 43.948Å b: 43.948Å c: 207.273Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.174 0.173 0.198
Expression system: Escherichia coli

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Citations

1 review citation

NMR Spectroscopy of supramolecular chemistry on protein surfaces.
Bayer et al. (2020)
4 other articles