› 6ss4

X-ray diffraction
2.9Å resolution

Function and Biology Details

Reaction catalysed: 
L-arginine + H(2)O = L-ornithine + urea
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211525 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Arginase-2, mitochondrial Chain: AAA
Molecule details ›
Chain: AAA
Length: 339 amino acids
Theoretical weight: 37.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P78540 (Residues: 23-354; Coverage: 94%)
Gene name: ARG2
Sequence domains: Arginase family
Fab C0021181 heavy chain (IgG1) Chain: HHH
Molecule details ›
Chain: HHH
Length: 233 amino acids
Theoretical weight: 24.82 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Fab C0021181 light chain (IgG1) Chain: LLL
Molecule details ›
Chain: LLL
Length: 220 amino acids
Theoretical weight: 23.12 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus

Ligands and Environments

2 bound ligands:
Ligand MN 2 x MN
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
R-values:
R R work R free
0.268 0.266 0.318
Expression systems:
  • Escherichia coli BL21(DE3)
  • Cricetulus griseus

Quick links

Citations

2 citation in other articles

Affinity maturation: highlights in the application of in vitro strategies for the directed evolution of antibodies.
Chan et al. (2021)
1 more

4 mentions without citation

Extensive sequence and structural evolution of Arginase 2 inhibitory antibodies enabled by an unbiased approach to affinity maturation.
Chan et al. (2020)
3 more