› 6sqz

X-ray diffraction
1.9Å resolution

Mouse dCTPase in complex with dCMPNPP

Released:
DOI: 10.2210/pdb6sqz/pdb
PDB entry 6sqz coloured by chain, front view.
PDB entry 6sqz coloured by chain, side view.
PDB entry 6sqz coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The First Structure of an Active Mammalian dCTPase and its Complexes With Substrate Analogs and Products.
Scaletti E, Claesson M, Helleday T, Jemth AS, Stenmark P
J Mol Biol 432 1126-1142 (2020)
PMID: 31954130

Function and Biology Details

Reaction catalysed: 
dCTP + H(2)O = dCMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193049 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
dCTP pyrophosphatase 1 Chains: A, D
Molecule details ›
Chains: A, D
Length: 170 amino acids
Theoretical weight: 18.79 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9QY93 (Residues: 1-170; Coverage: 100%)
Gene names: Dctpp1, Tdrg-TL1
Sequence domains: MazG-like family

Ligands and Environments

2 bound ligands:
Ligand MG 4 x MG
Ligand 0KX 2 x 0KX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P41212
Unit cell:
a: 58.997Å b: 58.997Å c: 142.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.187 0.217
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

DCTPP1, an Oncogene Regulated by miR-378a-3p, Promotes Proliferation of Breast Cancer via DNA Repair Signaling Pathway.
Niu et al. (2021)