› 6sbv

X-ray diffraction
2.6Å resolution

X-ray Structure of Human LDH-A with an Allosteric Inhibitor (Compound 7)

Released:
DOI: 10.2210/pdb6sbv/pdb
PDB entry 6sbv coloured by chain, front view.
PDB entry 6sbv coloured by chain, side view.
PDB entry 6sbv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Evidence for Isoform-Selective Allosteric Inhibition of Lactate Dehydrogenase A.
Friberg A, Rehwinkel H, Nguyen D, Pütter V, Quanz M, Weiske J, Eberspächer U, Heisler I, Langer G
ACS Omega 5 13034-13041 (2020)
PMID: 32548488

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132412 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase A chain Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 332 amino acids
Theoretical weight: 36.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00338 (Residues: 2-332; Coverage: 100%)
Gene names: LDHA, PIG19
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand L5K 8 x L5K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 80.369Å b: 146.287Å c: 308.362Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.219 0.242
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Targeting cancer metabolism in the era of precision oncology.
Stine et al. (2022)
1 more

1 mention without citation

β-Boswellic Acid Suppresses Breast Precancerous Lesions via GLUT1 Targeting-Mediated Glycolysis Inhibition and AMPK Pathway Activation.
Bie et al. (2022)