› 6rue

X-ray diffraction
1.65Å resolution

Wolinella succinogenes L-asparaginase mutant V23Q,K24T with L-Asp

Released:
DOI: 10.2210/pdb6rue/pdb
PDB entry 6rue coloured by chain, front view.
PDB entry 6rue coloured by chain, side view.
PDB entry 6rue coloured by chain, top view.
Source organism: Wolinella succinogenes DSM 1740
Primary publication:
Features of the Three Dimensional Structure of the Mutant Form of Wolinella succinogenes L-Asparaginase in Complexes with L-Aspartic and L-Glutamic Acids
Timofeev VI, Zhukhlistova NE, Kuranova IP
Russ. J. Bioorganic Chem. 46 (2020)

Function and Biology Details

Reaction catalysed: 
L-asparagine + H(2)O = L-aspartate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156162 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
L-asparaginase Chain: A
Molecule details ›
Chain: A
Length: 318 amino acids
Theoretical weight: 33.69 KDa
Source organism: Wolinella succinogenes DSM 1740
Expression system: Escherichia coli
UniProt:
  • Canonical: P50286 (Residues: 3-330; Coverage: 96%)
Gene names: WS0660, ansA, ansB
Sequence domains:
L-asparaginase Chain: B
Molecule details ›
Chain: B
Length: 314 amino acids
Theoretical weight: 33.4 KDa
Source organism: Wolinella succinogenes DSM 1740
Expression system: Escherichia coli
UniProt:
  • Canonical: P50286 (Residues: 3-330; Coverage: 95%)
Gene names: WS0660, ansA, ansB
Sequence domains:
L-asparaginase Chain: C
Molecule details ›
Chain: C
Length: 315 amino acids
Theoretical weight: 33.47 KDa
Source organism: Wolinella succinogenes DSM 1740
Expression system: Escherichia coli
UniProt:
  • Canonical: P50286 (Residues: 3-330; Coverage: 96%)
Gene names: WS0660, ansA, ansB
Sequence domains:
L-asparaginase Chain: D
Molecule details ›
Chain: D
Length: 317 amino acids
Theoretical weight: 33.62 KDa
Source organism: Wolinella succinogenes DSM 1740
Expression system: Escherichia coli
UniProt:
  • Canonical: P50286 (Residues: 3-330; Coverage: 96%)
Gene names: WS0660, ansA, ansB
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ASP 4 x ASP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 64.92Å b: 134.36Å c: 73.15Å
α: 90° β: 93.7° γ: 90°
R-values:
R R work R free
0.171 0.169 0.2
Expression system: Escherichia coli

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