› 6qwe

X-ray diffraction
1.4Å resolution

Crystal structure of KPC-4

Released:
DOI: 10.2210/pdb6qwe/pdb
PDB entry 6qwe coloured by chain, front view.
PDB entry 6qwe coloured by chain, side view.
PDB entry 6qwe coloured by chain, top view.
Source organism: Klebsiella pneumoniae
Primary publication:
Molecular Basis of Class A β-Lactamase Inhibition by Relebactam.
Tooke CL, Hinchliffe P, Lang PA, Mulholland AJ, Brem J, Schofield CJ, Spencer J
Antimicrob Agents Chemother 63 (2019)
PMID: 31383664

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190259 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbapenem-hydrolyzing beta-lactamase KPC Chain: A
Molecule details ›
Chain: A
Length: 290 amino acids
Theoretical weight: 30.82 KDa
Source organism: Klebsiella pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9F663 (Residues: 25-293; Coverage: 100%)
Gene names: bla, kpc, kpc1
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P21212
Unit cell:
a: 59.929Å b: 78.737Å c: 56.079Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.147 0.145 0.177
Expression system: Escherichia coli

Quick links

Citations

8 review citations

New β-Lactam-β-Lactamase Inhibitor Combinations.
Yahav et al. (2020)
7 more

2 mentions without citation

Molecular Basis of Class A β-Lactamase Inhibition by Relebactam.
Tooke et al. (2019)
1 more