› 6qrs

X-ray diffraction
1.17Å resolution

X-ray radiation dose series on xylose isomerase - 0.13 MGy

Released:
DOI: 10.2210/pdb6qrs/pdb
PDB entry 6qrs coloured by chain, front view.
PDB entry 6qrs coloured by chain, side view.
PDB entry 6qrs coloured by chain, top view.
Source organism: Streptomyces rubiginosus
Primary publication:
Structural knowledge or X-ray damage? A case study on xylose isomerase illustrating both.
Taberman H, Bury CS, van der Woerd MJ, Snell EH, Garman EF
J Synchrotron Radiat 26 931-944 (2019)
PMID: 31274415

Function and Biology Details

Reaction catalysed: 
Alpha-D-xylopyranose = alpha-D-xylufuranose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150204 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Xylose isomerase Chain: A
Molecule details ›
Chain: A
Length: 388 amino acids
Theoretical weight: 43.2 KDa
Source organism: Streptomyces rubiginosus
Expression system: Escherichia coli
UniProt:
  • Canonical: P24300 (Residues: 1-388; Coverage: 100%)
Gene name: xylA
Sequence domains: Xylose isomerase-like TIM barrel

Ligands and Environments

5 bound ligands:
Ligand EDO 12 x EDO
Ligand IPA 1 x IPA
Ligand MN 2 x MN
Ligand MG 2 x MG
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: I222
Unit cell:
a: 92.501Å b: 97.489Å c: 102.503Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.1 0.099 0.117
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Serial synchrotron and XFEL crystallography for studies of metalloprotein catalysis.
Hough et al. (2021)
5 other articles

1 mention without citation

Structural knowledge or X-ray damage? A case study on xylose isomerase illustrating both.
Taberman et al. (2019)