› 6qlk

X-ray diffraction
1.78Å resolution

Crystal structure of F181H UbiX in complex with prFMN

Released:
DOI: 10.2210/pdb6qlk/pdb
PDB entry 6qlk coloured by chain, front view.
PDB entry 6qlk coloured by chain, side view.
PDB entry 6qlk coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
The UbiX flavin prenyltransferase reaction mechanism resembles class I terpene cyclase chemistry.
Marshall SA, Payne KAP, Fisher K, White MD, Ní Cheallaigh A, Balaikaite A, Rigby SEJ, Leys D
Nat Commun 10 2357 (2019)
PMID: 31142738

Function and Biology Details

Reaction catalysed: 
Prenyl phosphate + FMNH(2) = prenylated FMNH(2) + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100427 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flavin prenyltransferase UbiX Chain: A
Molecule details ›
Chain: A
Length: 230 amino acids
Theoretical weight: 24.79 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A072ZCW8 (Residues: 1-209; Coverage: 100%)
Gene names: CAZ10_26235, DT376_15100, GNQ48_15570, GUL26_00020, IPC1295_14740, IPC737_17625, PAERUG_P19_London_7_VIM_2_05_10_06717, ubiX
Sequence domains: Flavoprotein

Ligands and Environments


Cofactor: Ligand 4LU 1 x 4LU
2 bound ligands:
Ligand PO4 1 x PO4
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: F23
Unit cell:
a: 141.751Å b: 141.751Å c: 141.751Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.188 0.212
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Bacterial terpenome.
Rudolf et al. (2021)
4 more

1 mention without citation

The UbiX flavin prenyltransferase reaction mechanism resembles class I terpene cyclase chemistry.
Marshall et al. (2019)