› 6qlf

Electron Microscopy
3.45Å resolution

Structure of inner kinetochore CCAN complex with mask1

Released:
DOI: 10.2210/pdb6qlf/pdb
PDB entry 6qlf coloured by chain, front view.
PDB entry 6qlf coloured by chain, side view.
PDB entry 6qlf coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of the inner kinetochore CCAN complex assembled onto a centromeric nucleosome.
Yan K, Yang J, Zhang Z, McLaughlin SH, Chang L, Fasci D, Ehrenhofer-Murray AE, Heck AJR, Barford D
Nature 574 278-282 (2019)
PMID: 31578520
Related structures: EMD-4581

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153600 (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
Inner kinetochore subunit IML3 Chain: L
Molecule details ›
Chain: L
Length: 245 amino acids
Theoretical weight: 28.09 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P38265 (Residues: 1-245; Coverage: 100%)
Gene names: IML3, MCM19, YBR0836, YBR107C
Inner kinetochore subunit CHL4 Chain: N
Molecule details ›
Chain: N
Length: 464 amino acids
Theoretical weight: 53.54 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P38907 (Residues: 1-458; Coverage: 100%)
Gene names: CHL4, CTF17, MCM17, YD9320A.04, YDR254W
Sequence domains: Kinetochore protein CHL4 like
Inner kinetochore subunit MCM21 Chain: O
Molecule details ›
Chain: O
Length: 368 amino acids
Theoretical weight: 43.03 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q06675 (Residues: 1-368; Coverage: 100%)
Gene names: CTF5, MCM21, YDR318W
Sequence domains: Cenp-O kinetochore centromere component
Inner kinetochore subunit CTF19 Chain: P
Molecule details ›
Chain: P
Length: 369 amino acids
Theoretical weight: 42.84 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q02732 (Residues: 1-369; Coverage: 100%)
Gene names: CTF19, LPB13W, MCM18, YPL018W
Inner kinetochore subunit OKP1 Chain: Q
Molecule details ›
Chain: Q
Length: 406 amino acids
Theoretical weight: 47.43 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P53298 (Residues: 1-406; Coverage: 100%)
Gene names: OKP1, YGR179C
Inner kinetochore subunit AME1 Chain: U
Molecule details ›
Chain: U
Length: 320 amino acids
Theoretical weight: 37.08 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P38313 (Residues: 1-320; Coverage: 99%)
Gene names: AME1, ARP100, YBR1458, YBR211C
Sequence domains: CENPU protein
Inner kinetochore subunit NKP1 Chain: Y
Molecule details ›
Chain: Y
Length: 238 amino acids
Theoretical weight: 27.01 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q12493 (Residues: 1-238; Coverage: 100%)
Gene names: NKP1, YDR383C
Inner kinetochore subunit NKP2 Chain: Z
Molecule details ›
Chain: Z
Length: 153 amino acids
Theoretical weight: 17.88 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q06162 (Residues: 1-153; Coverage: 100%)
Gene names: NKP2, YLR315W
Sequence domains: Cnl2/NKP2 family protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.45Å
Relevant EMDB volumes: EMD-4581
Expression system: Trichoplusia ni

Quick links

Citations

13 review citations

Principles of nucleosome recognition by chromatin factors and enzymes.
McGinty et al. (2021)
12 more

2 mentions without citation

Structure of the inner kinetochore CCAN complex assembled onto a centromeric nucleosome.
Yan et al. (2019)
1 more