› 6pwq

X-ray diffraction
2.6Å resolution

Function and Biology Details

Reaction catalysed: 
Sucrose + (6)-beta-D-fructofuranosyl-(2->)(n) alpha-D-glucopyranoside = glucose + (6)-beta-D-fructofuranosyl-(2->)(n+1) alpha-D-glucopyranoside
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Levansucrase Chains: A, B
Molecule details ›
Chains: A, B
Length: 466 amino acids
Theoretical weight: 52.45 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P05655 (Residues: 30-473; Coverage: 100%)
Gene names: BSU34450, sacB
Sequence domains: Levansucrase/Invertase

Ligands and Environments

4 bound ligands:
Ligand CA 2 x CA
Ligand SO4 20 x SO4
Ligand PG4 2 x PG4
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P43212
Unit cell:
a: 109.535Å b: 109.535Å c: 204.468Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.239
Expression system: Escherichia coli BL21

Quick links

Citations

7 citation in other articles

The molecular basis of the nonprocessive elongation mechanism in levansucrases.
Raga-Carbajal et al. (2021)
6 more