› 6p4d

X-ray diffraction
1.05Å resolution

Hen egg lysozyme (HEL) containing three point mutations (HEL3x): R21Q, R73E, and D101R

Released:
DOI: 10.2210/pdb6p4d/pdb
PDB entry 6p4d coloured by chain, front view.
PDB entry 6p4d coloured by chain, side view.
PDB entry 6p4d coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Conformational diversity facilitates antibody mutation trajectories and discrimination between foreign and self-antigens.
Burnett DL, Schofield P, Langley DB, Jackson J, Bourne K, Wilson E, Porebski BT, Buckle AM, Brink R, Goodnow CC, Christ D
Proc Natl Acad Sci U S A 117 22341-22350 (2020)
PMID: 32855302

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysozyme C Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.32 KDa
Source organism: Gallus gallus
Expression system: Homo sapiens
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family

Ligands and Environments

3 bound ligands:
Ligand CL 4 x CL
Ligand NA 1 x NA
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P43212
Unit cell:
a: 77.55Å b: 77.55Å c: 37.77Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.123 0.122 0.145
Expression system: Homo sapiens

Quick links

Citations

5 review citations

Insights Into the Host Contribution of Endocrine Associated Immune-Related Adverse Events to Immune Checkpoint Inhibition Therapy.
Chye et al. (2022)
4 more

1 mention without citation

Conformational diversity facilitates antibody mutation trajectories and discrimination between foreign and self-antigens.
Burnett et al. (2020)