› 6p27

X-ray diffraction
1.59Å resolution

Structure of a nested set of N-terminally extended MHC I-peptides provides novel insights into antigen processing and presentation

Released:
DOI: 10.2210/pdb6p27/pdb
PDB entry 6p27 coloured by chain, front view.
PDB entry 6p27 coloured by chain, side view.
PDB entry 6p27 coloured by chain, top view.
Source organisms:
Primary publication:
ERAP1 enzyme-mediated trimming and structural analyses of MHC I-bound precursor peptides yield novel insights into antigen processing and presentation.
Li L, Batliwala M, Bouvier M
J Biol Chem 294 18534-18544 (2019)
PMID: 31601650

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135237 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class I histocompatibility antigen, B alpha chain Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01889 (Residues: 25-300; Coverage: 82%)
Gene names: HLA-B, HLAB
Sequence domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
MHC I-peptide Chain: C
Molecule details ›
Chain: C
Length: 12 amino acids
Theoretical weight: 1.4 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MAA 1 x MAA

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 50.607Å b: 81.318Å c: 110.728Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.192 0.213
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Understanding the Pathogenesis of Spondyloarthritis.
Sharip et al. (2020)
2 more

2 mentions without citation

ERAP1 enzyme-mediated trimming and structural analyses of MHC I-bound precursor peptides yield novel insights into antigen processing and presentation.
Li et al. (2019)
1 more