› 6owy

X-ray diffraction
2.07Å resolution

Spy H96L:Im7 K20pI-Phe complex; multiple anomalous datasets contained herein for element identification

Released:
DOI: 10.2210/pdb6owy/pdb
PDB entry 6owy coloured by chain, front view.
PDB entry 6owy coloured by chain, side view.
PDB entry 6owy coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Identifying dynamic, partially occupied residues using anomalous scattering.
Rocchio S, Duman R, El Omari K, Mykhaylyk V, Orr C, Yan Z, Salmon L, Wagner A, Bardwell JCA, Horowitz S
Acta Crystallogr D Struct Biol 75 1084-1095 (2019)
PMID: 31793902

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160195 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Periplasmic chaperone Spy Chains: A, B
Molecule details ›
Chains: A, B
Length: 97 amino acids
Theoretical weight: 11.52 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P77754 (Residues: 52-147; Coverage: 70%)
Gene names: JW1732, b1743, spy
Sequence domains: LTXXQ motif family protein
Structure domains: Four Helix Bundle (Hemerythrin (Met), subunit A)

Ligands and Environments

4 bound ligands:
Ligand ZN 11 x ZN
Ligand CL 2 x CL
Ligand IMD 3 x IMD
Ligand IOD 1 x IOD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I23
Spacegroup: P4122
Unit cell:
a: 42.84Å b: 42.84Å c: 257.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.227 0.273
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Ion permeation in potassium ion channels.
Coates L. (2020)
1 more

1 mention without citation

Identifying dynamic, partially occupied residues using anomalous scattering.
Rocchio et al. (2019)