› 6oso

X-ray diffraction
1.75Å resolution

The crystal structure of the isolate tryptophan synthase alpha-chain from Salmonella enterica serovar typhimurium at 1.75 Angstrom resolution

Released:
DOI: 10.2210/pdb6oso/pdb
PDB entry 6oso coloured by chain, front view.
PDB entry 6oso coloured by chain, side view.
PDB entry 6oso coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Primary publication:
Backbone assignments and conformational dynamics in the S. typhimurium tryptophan synthase α-subunit from solution-state NMR.
Sakhrani VV, Hilario E, Caulkins BG, Hatcher-Skeers ME, Fan L, Dunn MF, Mueller LJ
J Biomol NMR 74 341-354 (2020)
PMID: 32415580

Function and Biology Details

Reaction catalysed: 
(1a) 1-C-(indol-3-yl)glycerol 3-phosphate = indole + D-glyceraldehyde 3-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133695 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tryptophan synthase alpha chain Chain: A
Molecule details ›
Chain: A
Length: 268 amino acids
Theoretical weight: 28.7 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00929 (Residues: 1-268; Coverage: 100%)
Gene names: STM1727, trpA
Sequence domains: Tryptophan synthase alpha chain

Ligands and Environments

3 bound ligands:
Ligand SO4 8 x SO4
Ligand CL 6 x CL
Ligand DMS 1 x DMS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P6522
Unit cell:
a: 70.94Å b: 70.94Å c: 203.401Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.172 0.171 0.204
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Allosteric regulation of substrate channeling: Salmonella typhimurium tryptophan synthase.
Ghosh et al. (2022)
2 other articles

8 mentions without citation

Galactosaminoglycans: Medical Applications and Drawbacks.
Pomin et al. (2019)
7 more