› 6os7

X-ray diffraction
1.36Å resolution

E. coli fumarase mutant - R126A

Released:
DOI: 10.2210/pdb6os7/pdb
PDB entry 6os7 coloured by chain, front view.
PDB entry 6os7 coloured by chain, side view.
PDB entry 6os7 coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Stuttgen GM, May JF, Bhattcharyya B, Weaver TM

Function and Biology Details

Reaction catalysed: 
(S)-malate = fumarate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138335 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fumarate hydratase class II Chains: A, B
Molecule details ›
Chains: A, B
Length: 472 amino acids
Theoretical weight: 51.15 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P05042 (Residues: 1-467; Coverage: 100%)
Gene names: JW1603, b1611, fumC
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand FLC 2 x FLC
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2221
Unit cell:
a: 103.521Å b: 217.598Å c: 86.266Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.146 0.145 0.169
Expression system: Escherichia coli K-12

Quick links