› 6os2

X-ray diffraction
2.7Å resolution

Structure of synthetic nanobody-stabilized angiotensin II type 1 receptor bound to TRV026

Released:
DOI: 10.2210/pdb6os2/pdb
PDB entry 6os2 coloured by chain, front view.
PDB entry 6os2 coloured by chain, side view.
PDB entry 6os2 coloured by chain, top view.
Source organisms:
Primary publication:
Angiotensin and biased analogs induce structurally distinct active conformations within a GPCR.
Wingler LM, Skiba MA, McMahon C, Staus DP, Kleinhenz ALW, Suomivuori CM, Latorraca NR, Dror RO, Lefkowitz RJ, Kruse AC
Science 367 888-892 (2020)
PMID: 32079768

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-231736 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Soluble cytochrome b562; Type-1 angiotensin II receptor Chain: A
Molecule details ›
Chain: A
Length: 425 amino acids
Theoretical weight: 48.51 KDa
Source organisms: Expression system: Homo sapiens
UniProt:
  • Canonical: P30556 (Residues: 2-226, 227-319; Coverage: 89%)
  • Canonical: P0ABE7 (Residues: 24-122; Coverage: 93%)
Gene names: AGTR1, AGTR1A, AGTR1B, AT2R1, AT2R1B, cybC
Sequence domains:
Nanobody Nb.AT110i1_le Chain: D
Molecule details ›
Chain: D
Length: 128 amino acids
Theoretical weight: 13.8 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
TRV026 peptide Chain: B
Molecule details ›
Chain: B
Length: 8 amino acids
Theoretical weight: 905 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand NAG 1 x NAG
Ligand OLC 6 x OLC
Ligand CLR 1 x CLR
1 modified residue:
Ligand SAR 1 x SAR

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: I222
Unit cell:
a: 59.96Å b: 101.35Å c: 225.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.233 0.285
Expression systems:
  • Homo sapiens
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

22 review citations

Conformational Basis of G Protein-Coupled Receptor Signaling Versatility.
Wingler et al. (2020)
21 more

13 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
12 more