› 6omm

Electron Microscopy
3.17Å resolution

Cryo-EM structure of formyl peptide receptor 2/lipoxin A4 receptor in complex with Gi

Released:
DOI: 10.2210/pdb6omm/pdb
PDB entry 6omm coloured by chain, front view.
PDB entry 6omm coloured by chain, side view.
PDB entry 6omm coloured by chain, top view.
Source organisms:
Primary publication:
Structure of formylpeptide receptor 2-Gi complex reveals insights into ligand recognition and signaling.
Zhuang Y, Liu H, Edward Zhou X, Kumar Verma R, de Waal PW, Jang W, Xu TH, Wang L, Meng X, Zhao G, Kang Y, Melcher K, Fan H, Lambert NA, Eric Xu H, Zhang C
Nat Commun 11 885 (2020)
PMID: 32060286
Related structures: EMD-20126

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-210882 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
N-formyl peptide receptor 2 Chain: R
Molecule details ›
Chain: R
Length: 363 amino acids
Theoretical weight: 40.33 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P25090 (Residues: 1-342; Coverage: 97%)
Gene names: FPR2, FPRH1, FPRL1, LXA4R
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Peptide agonist Chain: L
Molecule details ›
Chain: L
Length: 6 amino acids
Theoretical weight: 857 Da
Source organism: synthetic construct
Expression system: Not provided
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 353 amino acids
Theoretical weight: 40.33 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 2-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 353 amino acids
Theoretical weight: 39.02 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 67 amino acids
Theoretical weight: 7.43 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 2-68; Coverage: 94%)
Gene name: GNG2
Sequence domains: GGL domain
scFv16 Chain: E
Molecule details ›
Chain: E
Length: 247 amino acids
Theoretical weight: 26.32 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand CLR 5 x CLR
Ligand PLM 3 x PLM
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.17Å
Relevant EMDB volumes: EMD-20126
Expression systems:
  • Spodoptera frugiperda
  • Not provided
  • Escherichia coli

Quick links

Citations

9 review citations

Pro-resolving lipid mediators: regulators of inflammation, metabolism and kidney function.
Brennan et al. (2021)
8 more

21 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
20 more