› 6ol9

X-ray diffraction
2.54Å resolution

Structure of the M5 muscarinic acetylcholine receptor (M5-T4L) bound to tiotropium

Released:
DOI: 10.2210/pdb6ol9/pdb
PDB entry 6ol9 coloured by chain, front view.
PDB entry 6ol9 coloured by chain, side view.
PDB entry 6ol9 coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of the M5 muscarinic acetylcholine receptor.
Vuckovic Z, Gentry PR, Berizzi AE, Hirata K, Varghese S, Thompson G, van der Westhuizen ET, Burger WAC, Rahmani R, Valant C, Langmead CJ, Lindsley CW, Baell JB, Tobin AB, Sexton PM, Christopoulos A, Thal DM
Proc Natl Acad Sci U S A 116 26001-26007 (2019)
PMID: 31772027

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112933 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin; Muscarinic acetylcholine receptor M5 Chain: A
Molecule details ›
Chain: A
Length: 480 amino acids
Theoretical weight: 55.09 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P08912 (Residues: 21-224, 431-532; Coverage: 58%)
  • Canonical: D9IEF7 (Residues: 2-161; Coverage: 98%)
Gene names: CHRM5, T4Tp126, e
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand OLA 2 x OLA
Ligand 0HK 1 x 0HK
Ligand P33 1 x P33
Ligand OLC 1 x OLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: C2
Unit cell:
a: 97.06Å b: 63.31Å c: 91.62Å
α: 90° β: 102.11° γ: 90°
R-values:
R R work R free
0.237 0.236 0.257
Expression system: Spodoptera frugiperda

Quick links

Citations

8 review citations

Targeting Muscarinic Acetylcholine Receptors for the Treatment of Psychiatric and Neurological Disorders.
Moran et al. (2019)
7 more

7 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
6 more