› 6ok4

X-ray diffraction
2.4Å resolution

Crystal Structure of Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from Chlamydia trachomatis with bound NAD

Released:
DOI: 10.2210/pdb6ok4/pdb
PDB entry 6ok4 coloured by chain, front view.
PDB entry 6ok4 coloured by chain, side view.
PDB entry 6ok4 coloured by chain, top view.
Source organism: Chlamydia trachomatis D/UW-3/CX
Primary publication:
Structures of glyceraldehyde 3-phosphate dehydrogenase in Neisseria gonorrhoeae and Chlamydia trachomatis.
Barrett KF, Dranow DM, Phan IQ, Michaels SA, Shaheen S, Navaluna ED, Craig JK, Tillery LM, Choi R, Edwards TE, Conrady DG, Abendroth J, Horanyi PS, Lorimer DD, Van Voorhis WC, Zhang Z, Barrett LK, Subramanian S, Staker B, Fan E, Myler PJ, Soge OO, Hybiske K, Ojo KK
Protein Sci 29 768-778 (2020)
PMID: 31930578

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143206 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glyceraldehyde-3-phosphate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 342 amino acids
Theoretical weight: 37.36 KDa
Source organism: Chlamydia trachomatis D/UW-3/CX
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0CE13 (Residues: 1-334; Coverage: 100%)
Gene names: CT_505, gap, gapA
Sequence domains:
Structure domains: Dihydrodipicolinate Reductase; domain 2

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
4 bound ligands:
Ligand EDO 15 x EDO
Ligand PO4 6 x PO4
Ligand PEG 4 x PEG
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 86.47Å b: 135.29Å c: 137.67Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.208
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Epidemiology, Treatments, and Vaccine Development for Antimicrobial-Resistant Neisseria gonorrhoeae: Current Strategies and Future Directions.
Lin et al. (2021)
1 more

3 mentions without citation

Structures of glyceraldehyde 3-phosphate dehydrogenase in Neisseria gonorrhoeae and Chlamydia trachomatis.
Barrett et al. (2020)
2 more