› 6oe5

X-ray diffraction
4.1Å resolution

Splayed open prefusion RSV F captured by CR9501 and motavizumab Fabs

Released:
DOI: 10.2210/pdb6oe5/pdb
PDB entry 6oe5 coloured by chain, front view.
PDB entry 6oe5 coloured by chain, side view.
PDB entry 6oe5 coloured by chain, top view.
Source organisms:
Primary publication:
Transient opening of trimeric prefusion RSV F proteins.
Gilman MSA, Furmanova-Hollenstein P, Pascual G, B van 't Wout A, Langedijk JPM, McLellan JS
Nat Commun 10 2105 (2019)
PMID: 31068578

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208917 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Fibritin; Fusion glycoprotein F0 Chain: A
Molecule details ›
Chain: A
Length: 553 amino acids
Theoretical weight: 61.37 KDa
Source organisms: Expression system: Homo sapiens
UniProt:
  • Canonical: P03420 (Residues: 1-513; Coverage: 89%)
  • Canonical: P10104 (Residues: 458-484; Coverage: 6%)
Gene names: F, wac
Sequence domains: Fusion glycoprotein F0
Motavizumab Fab Heavy Chain Chain: B
Molecule details ›
Chain: B
Length: 225 amino acids
Theoretical weight: 24.28 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Motavizumab Fab Light Chain Chain: C
Molecule details ›
Chain: C
Length: 213 amino acids
Theoretical weight: 23.15 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
CR9501 Fab Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 230 amino acids
Theoretical weight: 24.37 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
CR9501 Fab Light Chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.56 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P61
Unit cell:
a: 219.837Å b: 219.837Å c: 68.811Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.264 0.262 0.306
Expression system: Homo sapiens

Quick links

Citations

8 review citations

Current State of Respiratory Syncytial Virus Disease and Management.
Chatterjee et al. (2021)
7 more

3 mentions without citation

Antibody Epitopes of Pneumovirus Fusion Proteins.
Huang et al. (2019)
2 more