› 6obl

X-ray diffraction
2.06Å resolution

JAK2 JH2 in complex with JAK168

Released:
DOI: 10.2210/pdb6obl/pdb
PDB entry 6obl coloured by chain, front view.
PDB entry 6obl coloured by chain, side view.
PDB entry 6obl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Selective Janus Kinase 2 (JAK2) Pseudokinase Ligands with a Diaminotriazole Core.
Liosi ME, Krimmer SG, Newton AS, Dawson TK, Puleo DE, Cutrona KJ, Suzuki Y, Schlessinger J, Jorgensen WL
J Med Chem 63 5324-5340 (2020)
PMID: 32329617

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130063 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein kinase JAK2 Chain: A
Molecule details ›
Chain: A
Length: 289 amino acids
Theoretical weight: 33.12 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O60674 (Residues: 536-812; Coverage: 25%)
Gene name: JAK2
Sequence domains: Protein tyrosine and serine/threonine kinase

Ligands and Environments

1 bound ligand:
Ligand M4P 1 x M4P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 43.605Å b: 57.782Å c: 61.009Å
α: 90° β: 110.71° γ: 90°
R-values:
R R work R free
0.2 0.198 0.239
Expression system: Spodoptera frugiperda

Quick links

Citations

3 review citations

There's more to death than life: Noncatalytic functions in kinase and pseudokinase signaling.
Mace et al. (2021)
2 more

1 mention without citation

Selective Janus Kinase 2 (JAK2) Pseudokinase Ligands with a Diaminotriazole Core.
Liosi et al. (2020)