› 6o56

X-ray diffraction
1.9Å resolution

HNH Nuclease from S. pyogenes Cas9

Released:
DOI: 10.2210/pdb6o56/pdb
PDB entry 6o56 coloured by chain, front view.
PDB entry 6o56 coloured by chain, side view.
PDB entry 6o56 coloured by chain, top view.
Source organism: Streptococcus pyogenes serotype M1
Primary publication:
Allosteric Motions of the CRISPR-Cas9 HNH Nuclease Probed by NMR and Molecular Dynamics.
East KW, Newton JC, Morzan UN, Narkhede YB, Acharya A, Skeens E, Jogl G, Batista VS, Palermo G, Lisi GP
J Am Chem Soc 142 1348-1358 (2020)
PMID: 31885264

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189431 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR-associated endonuclease Cas9/Csn1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 135 amino acids
Theoretical weight: 15.99 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99ZW2 (Residues: 775-908; Coverage: 10%)
Gene names: SPy_1046, cas9, csn1
Sequence domains: HNH endonuclease

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-003
Spacegroup: P212121
Unit cell:
a: 51.525Å b: 56.063Å c: 80.669Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.253
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Spotlight on the Ballet of Proteins: The Structural Dynamic Properties of Proteins Illuminated by Solution NMR.
Tokunaga et al. (2020)
6 more

7 mentions without citation

Insights into the Mechanism of CRISPR/Cas9-Based Genome Editing from Molecular Dynamics Simulations.
Bhattacharya et al. (2023)
6 more