› 6o2w

X-ray diffraction
2.1Å resolution

Crystal structure of the SARAF luminal domain Cys-lock mutant dimer

Released:
DOI: 10.2210/pdb6o2w/pdb
PDB entry 6o2w coloured by chain, front view.
PDB entry 6o2w coloured by chain, side view.
PDB entry 6o2w coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
SARAF Luminal Domain Structure Reveals a Novel Domain-Swapped β-Sandwich Fold Important for SOCE Modulation.
Kimberlin CR, Meshcheriakova A, Palty R, Raveh A, Karbat I, Reuveny E, Minor DL
J Mol Biol 431 2869-2883 (2019)
PMID: 31082439

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188389 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Store-operated calcium entry-associated regulatory factor Chains: A, B
Molecule details ›
Chains: A, B
Length: 135 amino acids
Theoretical weight: 15.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96BY9 (Residues: 30-164; Coverage: 43%)
Gene names: HSPC035, NPD003, PSEC0019, SARAF, TMEM66, UNQ1967/PRO4499, XTP3
Sequence domains: SOCE-associated regulatory factor of calcium homoeostasis

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P1
Unit cell:
a: 24.91Å b: 30.79Å c: 76.14Å
α: 88.58° β: 86.03° γ: 89.79°
R-values:
R R work R free
0.178 0.175 0.234
Expression system: Escherichia coli

Quick links

Citations

5 review citations

More Than Just Simple Interaction between STIM and Orai Proteins: CRAC Channel Function Enabled by a Network of Interactions with Regulatory Proteins.
Berlansky et al. (2021)
4 more

1 mention without citation

SARAF Luminal Domain Structure Reveals a Novel Domain-Swapped β-Sandwich Fold Important for SOCE Modulation.
Kimberlin et al. (2019)