› 6nvh

X-ray diffraction
1.9Å resolution

FGFR4 complex with N-(2-((5-((2,6-dichloro-3,5-dimethoxybenzyl)oxy)pyrimidin-2-yl)amino)-3-methylphenyl)acrylamide

Released:
DOI: 10.2210/pdb6nvh/pdb
PDB entry 6nvh coloured by chain, front view.
PDB entry 6nvh coloured by chain, side view.
PDB entry 6nvh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Rotational Freedom, Steric Hindrance, and Protein Dynamics Explain BLU554 Selectivity for the Hinge Cysteine of FGFR4.
Lin X, Yosaatmadja Y, Kalyukina M, Middleditch MJ, Zhang Z, Lu X, Ding K, Patterson AV, Smaill JB, Squire CJ
ACS Med Chem Lett 10 1180-1186 (2019)
PMID: 31413803

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149636 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fibroblast growth factor receptor 4 Chain: A
Molecule details ›
Chain: A
Length: 300 amino acids
Theoretical weight: 33.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P22455 (Residues: 450-751; Coverage: 38%)
Gene names: FGFR4, JTK2, TKF
Sequence domains: Protein tyrosine and serine/threonine kinase

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand XL6 1 x XL6
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P43212
Unit cell:
a: 64.127Å b: 64.127Å c: 181.924Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.212 0.234
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Advances of Fibroblast Growth Factor/Receptor Signaling Pathway in Hepatocellular Carcinoma and its Pharmacotherapeutic Targets.
Wang et al. (2021)
4 other articles

1 mention without citation

Investigation of Covalent Warheads in the Design of 2-Aminopyrimidine-based FGFR4 Inhibitors.
Deng et al. (2021)