› 6nph

Electron Microscopy
2.9Å resolution

Structure of NKCC1 TM domain

Released:
DOI: 10.2210/pdb6nph/pdb
PDB entry 6nph coloured by chain, front view.
PDB entry 6nph coloured by chain, side view.
PDB entry 6nph coloured by chain, top view.
Source organism: Danio rerio
Primary publication:
Structure and mechanism of the cation-chloride cotransporter NKCC1.
Chew TA, Orlando BJ, Zhang J, Latorraca NR, Wang A, Hollingsworth SA, Chen DH, Dror RO, Liao M, Feng L
Nature 572 488-492 (2019)
PMID: 31367042
Related structures: EMD-0470

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101346 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Solute carrier family 12 member 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 472 amino acids
Theoretical weight: 50.62 KDa
Source organism: Danio rerio
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: A0A0G2KTI4 (Residues: 206-677; Coverage: 42%)
Gene names: nkcc1, slc12a2
Sequence domains: Amino acid permease

Ligands and Environments

3 bound ligands:
Ligand POV 7 x POV
Ligand K 2 x K
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-0470
Expression system: Spodoptera frugiperda

Quick links

Citations

15 review citations

Fluid transport in the brain.
Rasmussen et al. (2022)
14 more

5 mentions without citation

High-Resolution Views and Transport Mechanisms of the NKCC1 and KCC Transporters.
Chew et al. (2021)
4 more