› 6noh

X-ray diffraction
2.25Å resolution

Crystal structure of FBF-2 repeat 5 mutant (C363S, R364Y, Q367S) in complex with 8-nt RNA

Released:
DOI: 10.2210/pdb6noh/pdb
PDB entry 6noh coloured by chain, front view.
PDB entry 6noh coloured by chain, side view.
PDB entry 6noh coloured by chain, top view.
Source organism: Caenorhabditis elegans
Primary publication:
Engineering a conserved RNA regulatory protein repurposes its biological function in vivo.
Bhat VD, McCann KL, Wang Y, Fonseca DR, Shukla T, Alexander JC, Qiu C, Wickens M, Lo TW, Tanaka Hall TM, Campbell ZT
Elife 8 (2019)
PMID: 30652968

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170781 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Fem-3 mRNA-binding factor 2 Chain: A
Molecule details ›
Chain: A
Length: 413 amino acids
Theoretical weight: 46.97 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q09312 (Residues: 164-575; Coverage: 65%)
Gene names: F21H12.5, fbf-2
Sequence domains: Pumilio-family RNA binding repeat
Structure domains: Leucine-rich Repeat Variant
RNA (5'-R(*UP*GP*UP*AP*AP*AP*UP*A)-3') Chain: B
Molecule details ›
Chain: B
Length: 8 nucleotides
Theoretical weight: 2.54 KDa
Source organism: Caenorhabditis elegans
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand EDO 13 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P61
Unit cell:
a: 96.397Å b: 96.397Å c: 99.853Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.162 0.159 0.206
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Diverse Roles of PUF Proteins in Germline Stem and Progenitor Cell Development in C. elegans.
Wang et al. (2020)
2 more