› 6n7g

Electron Microscopy
6.8Å resolution

Cryo-EM structure of tetrameric Ptch1 in complex with ShhNp (form I)

Released:
DOI: 10.2210/pdb6n7g/pdb
PDB entry 6n7g coloured by chain, front view.
PDB entry 6n7g coloured by chain, side view.
PDB entry 6n7g coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Inhibition of tetrameric Patched1 by Sonic Hedgehog through an asymmetric paradigm.
Qian H, Cao P, Hu M, Gao S, Yan N, Gong X
Nat Commun 10 2320 (2019)
PMID: 31127104
Related structures: EMD-0355

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171801 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Protein patched homolog 1 Chains: A, B, D, E
Molecule details ›
Chains: A, B, D, E
Length: 1349 amino acids
Theoretical weight: 150.19 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q13635 (Residues: 1-1305; Coverage: 90%)
Gene names: PTCH, PTCH1
Sequence domains: Patched family
Sonic hedgehog protein N-product Chains: C, F
Molecule details ›
Chains: C, F
Length: 174 amino acids
Theoretical weight: 19.59 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q15465 (Residues: 24-197; Coverage: 40%)
Gene name: SHH
Sequence domains: Hedgehog amino-terminal signalling domain

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
5 bound ligands:
Ligand NAG 20 x NAG
Ligand CLR 10 x CLR
Ligand ZN 2 x ZN
Ligand CA 4 x CA
Ligand PLM 2 x PLM
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.8Å
Relevant EMDB volumes: EMD-0355
Expression system: Homo sapiens

Quick links

Citations

19 review citations

Structures of vertebrate Patched and Smoothened reveal intimate links between cholesterol and Hedgehog signalling.
Kowatsch et al. (2019)
18 more