› 6msi

X-ray diffraction
1.65Å resolution

TYPE III ANTIFREEZE PROTEIN ISOFORM HPLC 12

Released:
DOI: 10.2210/pdb6msi/pdb
PDB entry 6msi coloured by chain, front view.
PDB entry 6msi coloured by chain, side view.
PDB entry 6msi coloured by chain, top view.
Source organism: Zoarces americanus
Primary publication:
The effects of steric mutations on the structure of type III antifreeze protein and its interaction with ice.
DeLuca CI, Davies PL, Ye Q, Jia Z
J Mol Biol 275 515-25 (1998)
PMID: 9466928

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148668 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type-3 ice-structuring protein HPLC 12 Chain: A
Molecule details ›
Chain: A
Length: 66 amino acids
Theoretical weight: 7 KDa
Source organism: Zoarces americanus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19614 (Residues: 2-63; Coverage: 94%)
Structure domains: Antifreeze-like/N-acetylneuraminic acid synthase C-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 33.157Å b: 40.038Å c: 44.81Å
α: 90° β: 90° γ: 90°
Expression system: Escherichia coli

Quick links

Citations

8 review citations

'Antifreeze' glycoproteins from polar fish.
Harding et al. (2003)
7 more

4 mentions without citation

Thoroughly sampling sequence space: large-scale protein design of structural ensembles.
Larson et al. (2002)
3 more