› 6mnl

Solution NMR

NMR solution structures of second bromodomain of BRD4 with FOXO3a peptide

Released:
DOI: 10.2210/pdb6mnl/pdb
PDB entry 6mnl coloured by chain, ensemble of 20 models, front view.
PDB entry 6mnl coloured by chain, ensemble of 20 models, side view.
PDB entry 6mnl coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Targeting the BRD4/FOXO3a/CDK6 axis sensitizes AKT inhibition in luminal breast cancer.
Liu J, Duan Z, Guo W, Zeng L, Wu Y, Chen Y, Tai F, Wang Y, Lin Y, Zhang Q, He Y, Deng J, Stewart RL, Wang C, Lin PC, Ghaffari S, Evers BM, Liu S, Zhou MM, Zhou BP, Shi J
Nat Commun 9 5200 (2018)
PMID: 30518851

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128983 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Forkhead box protein O3 Chain: A
Molecule details ›
Chain: A
Length: 16 amino acids
Theoretical weight: 1.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43524 (Residues: 237-252; Coverage: 2%)
Gene names: FKHRL1, FOXO3, FOXO3A
Bromodomain-containing protein 4 Chain: B
Molecule details ›
Chain: B
Length: 128 amino acids
Theoretical weight: 14.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O60885 (Residues: 333-460; Coverage: 9%)
Gene names: BRD4, HUNK1
Sequence domains: Bromodomain
Structure domains: Bromodomain-like

Ligands and Environments

No bound ligands
1 modified residue:
Ligand ALY 2 x ALY

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 82%
Refinement method: torsion angle dynamics
Expression system: Escherichia coli

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Citations

16 review citations

Targeting Super-Enhancers as a Therapeutic Strategy for Cancer Treatment.
He et al. (2019)
15 more