› 6mm8

X-ray diffraction
1.85Å resolution

Catalytic subunit of cAMP-dependent protein kinase A in complex with RyR2 K2879A, S2813D phosphomimetic (2699-2904) crystal form 2

Released:
DOI: 10.2210/pdb6mm8/pdb
PDB entry 6mm8 coloured by chain, front view.
PDB entry 6mm8 coloured by chain, side view.
PDB entry 6mm8 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
cAMP-dependent protein kinase A in complex with RyR2 peptide (2799-2810)
Haji-Ghassemi O, Yuchi Z, van Petegem F
Mol. Cell (2019)

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123215 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
cAMP-dependent protein kinase catalytic subunit alpha Chain: C
Molecule details ›
Chain: C
Length: 339 amino acids
Theoretical weight: 39.6 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P05132 (Residues: 16-351; Coverage: 96%)
Gene names: Pkaca, Prkaca
Sequence domains: Protein kinase domain
Structure domains:
Ryanodine receptor 2 Chain: D
Molecule details ›
Chain: D
Length: 209 amino acids
Theoretical weight: 24.29 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: E9Q401 (Residues: 2699-2904; Coverage: 4%)
Gene name: Ryr2
Sequence domains: RyR domain
Structure domains: Globin-like

Ligands and Environments

5 bound ligands:
Ligand PEG 1 x PEG
Ligand ACY 2 x ACY
Ligand ANP 1 x ANP
Ligand EDO 5 x EDO
Ligand CL 1 x CL
2 modified residues:
Ligand TPO 1 x TPO
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C2
Unit cell:
a: 179.643Å b: 67.86Å c: 58.529Å
α: 90° β: 92.22° γ: 90°
R-values:
R R work R free
0.183 0.181 0.221
Expression system: Escherichia coli

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