› 6m83

X-ray diffraction
1.37Å resolution

Crystal structure of TylM1 S120A bound to SAH and dTDP-phenol

Released:
DOI: 10.2210/pdb6m83/pdb
PDB entry 6m83 coloured by chain, front view.
PDB entry 6m83 coloured by chain, side view.
PDB entry 6m83 coloured by chain, top view.
Source organism: Streptomyces fradiae
Primary publication:
Structural and Functional Characterization of Sulfonium Carbon-Oxygen Hydrogen Bonding in the Deoxyamino Sugar Methyltransferase TylM1.
Fick RJ, Horowitz S, McDole BG, Clay MC, Mehl RA, Al-Hashimi HM, Scheiner S, Trievel RC
Biochemistry 58 2152-2159 (2019)
PMID: 30810306

Function and Biology Details

Reaction catalysed: 
2 S-adenosyl-L-methionine + dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose = 2 S-adenosyl-L-homocysteine + dTDP-3-dimethylamino-3,6-dideoxy-alpha-D-glucopyranose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161176 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose N,N-dimethyltransferase Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 28.52 KDa
Source organism: Streptomyces fradiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P95748 (Residues: 1-255; Coverage: 100%)
Gene names: tylM1, tylMI
Sequence domains: Methyltransferase domain

Ligands and Environments


Cofactor: Ligand SAH 1 x SAH
2 bound ligands:
Ligand TLO 1 x TLO
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: I222
Unit cell:
a: 64.521Å b: 91.267Å c: 93.629Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.153 0.182
Expression system: Escherichia coli

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