› 6m2y

X-ray diffraction
2.1Å resolution

Crystal structure of a formolase, BFD variant M6 from Pseudomonas putida

Released:
DOI: 10.2210/pdb6m2y/pdb
PDB entry 6m2y coloured by chain, front view.
PDB entry 6m2y coloured by chain, side view.
PDB entry 6m2y coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Totally atom-economical synthesis of lactic acid from formaldehyde: combined bio-carboligation and chemo-rearrangement without the isolation of intermediate.
Li T, Tang Z, Wei H, Tan Z, Liu P, Li J, Zheng Y, Lin J, Liu W, Jiang H, Liu H, Zhu L, Ma Y
Green Chem 22 6809-6814 (2020)

Function and Biology Details

Reaction catalysed: 
Benzoylformate = benzaldehyde + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149084 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Benzoylformate decarboxylase Chain: A
Molecule details ›
Chain: A
Length: 528 amino acids
Theoretical weight: 56.5 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: P20906 (Residues: 1-528; Coverage: 100%)
Gene name: mdlC
Sequence domains:

Ligands and Environments


Cofactor: Ligand TPP 1 x TPP
1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: I222
Unit cell:
a: 103.515Å b: 106.674Å c: 116.548Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.154 0.189
Expression system: Escherichia coli

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