› 6lp2

X-ray diffraction
2.48Å resolution

Structure of Lpg2148/UBE2N-Ub complex

Released:
DOI: 10.2210/pdb6lp2/pdb
PDB entry 6lp2 coloured by chain, front view.
PDB entry 6lp2 coloured by chain, side view.
PDB entry 6lp2 coloured by chain, top view.
Source organisms:
Entry authors: Feng Y, Wang Y, Huang Y, Li D

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-143255 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Uncharacterized protein lpg2148 Chain: A
Molecule details ›
Chain: A
Length: 386 amino acids
Theoretical weight: 44.16 KDa
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5ZTL3 (Residues: 13-396; Coverage: 90%)
Gene name: lpg2148
Ubiquitin Chain: C
Molecule details ›
Chain: C
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG47 (Residues: 153-228; Coverage: 33%)
Gene name: UBB
Sequence domains: Ubiquitin family
Ubiquitin-conjugating enzyme E2 N Chain: D
Molecule details ›
Chain: D
Length: 152 amino acids
Theoretical weight: 17.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61088 (Residues: 1-152; Coverage: 100%)
Gene names: BLU, UBE2N
Sequence domains: Ubiquitin-conjugating enzyme

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: C2
Unit cell:
a: 139.953Å b: 71.266Å c: 75.111Å
α: 90° β: 90.74° γ: 90°
R-values:
R R work R free
0.178 0.175 0.233
Expression system: Escherichia coli

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