› 6ln2

X-ray diffraction
3.2Å resolution

Crystal structure of full length human GLP1 receptor in complex with Fab fragment (Fab7F38)

Released:
DOI: 10.2210/pdb6ln2/pdb
PDB entry 6ln2 coloured by chain, front view.
PDB entry 6ln2 coloured by chain, side view.
PDB entry 6ln2 coloured by chain, top view.
Source organisms:
Primary publication:
Full-length human GLP-1 receptor structure without orthosteric ligands.
Wu F, Yang L, Hang K, Laursen M, Wu L, Han GW, Ren Q, Roed NK, Lin G, Hanson MA, Jiang H, Wang MW, Reedtz-Runge S, Song G, Stevens RC
Nat Commun 11 1272 (2020)
PMID: 32152292

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208203 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Glucagon-like peptide 1 receptor; Rubredoxin Chain: A
Molecule details ›
Chain: A
Length: 469 amino acids
Theoretical weight: 54.62 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P43220 (Residues: 24-260, 262-439; Coverage: 94%)
  • Canonical: P00268 (Residues: 1-54; Coverage: 100%)
Gene name: GLP1R
Sequence domains:
Fab7F38_light chain Chain: B
Molecule details ›
Chain: B
Length: 213 amino acids
Theoretical weight: 23.31 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Fab7F38_heavy chain Chain: C
Molecule details ›
Chain: C
Length: 226 amino acids
Theoretical weight: 24.48 KDa
Source organism: Mus musculus
Expression system: Mus musculus

Ligands and Environments

3 bound ligands:
Ligand NAG 1 x NAG
Ligand ZN 1 x ZN
Ligand 97Y 1 x 97Y
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45XU
Spacegroup: P212121
Unit cell:
a: 63.04Å b: 64.66Å c: 322.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.213 0.262
Expression systems:
  • Spodoptera frugiperda
  • Mus musculus

Quick links

Citations

8 review citations

Mechanisms of adhesion G protein-coupled receptor activation.
Vizurraga et al. (2020)
7 more

18 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
17 more